And I heard a loud voice from the throne saying, “Now the dwelling of God is with men, and he will live with them. They will be his people, and God himself will be with them and be their God. He will wipe every tear from their eyes. There will be no more death or mourning or crying or pain, for the old order of things has passed away.” Revelation 21:3-4

No. The only tears will be tears of joy as we experience the fulfillment of Scripture, the forgiveness of sins, and the presence of God. There will be no more sin, no more regret, no more failure, no more disobedience, no more guilt, no more physical pain.